Start main content

CombiSEAL: New Pathfinder for New Protein Discovery

CombiSEAL: New Pathfinder for New Protein Discovery
Research Tools and Methods
Reference No.

Key Problem and Market Opportunity

  • Using site-directed mutagenesis for protein engineering to make better enzymes, antibodies, and protein drugs lacks throughput and scalability
  • Protein function not only depends on one or two amino acids but a combination of changes in many cases

Key Advantages of the Technology

Technology Section Slider 1
Technology Section Slider 2
  • Simultaneously study the interaction of different amino acids in a protein at a much faster speed
  • First platform for assembling and barcoding protein-encoding sequences carrying multiple mutations en masse
  • Coupled with next-generation sequencing to track all variants in an unprecedented throughput
  • Uses barcoded sequences to easily identify the functional variants
  • Helped to identify a better error-free Cas9 protein


Further Details

Choi GC, et al. Combinatorial mutagenesis en masse optimizes the genome editing activities of SpCas9. Nat Methods 16, 722–730 (2019).

Potential Product and Services

  • Drug discovery service
  • Reagent kits
  • Automatic platform to the combinatorial constructs

Development Status

  • PCT Application No.: PCT/CN2019/106096
IP Status
  • Patent application submitted

Work with us